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15921799099
在科學中共享試劑是??*的，并可以促進協作，但也可以促進發現并驗證過去的結果。這些步驟有助于推動科學向前發展。MRC PPU的研究人員已經發表了前沿科學多年，并且正如預期的那樣，廣泛要求他們論文中使用的工具來促進其他人的工作以及整個領域。
考慮到所需試劑的數量，我們在2013年創建了一個網站，以方便訪問數據庫中的這些研究工具。盡管我們初的投資組合開始專注于遍在蛋白系統中的激酶，磷酸酶和酶，但我們的科學家-因此我們的試劑-已擴展到新領域。我們歡迎您查看我們不斷擴大的試劑目錄，以找出可能有助于您自己的研究工作的試劑。
此外，我們很樂意為研究人員提供定制生成的蛋白質或抗體的幫助。迄今為止，我們已經與來自學術界以及制藥和生物技術領域的世界各地的研究人員合作完成了許多項目。請讓我們知道我們將如何幫助您！
感興趣的cDNA的克隆和亞克隆是分子生物學的基礎，并有助于隨后的生化和細胞生物學研究。盡管看似常規，但我們創建和驗證克隆的方法仍保持一定程度的嚴格性，以確保所有克隆均符合本單位科學家所要求的準確度標準。
通過測序驗證所有cDNA插入片段，然后將原始測序跡線與參考序列（Genbank）進行比對以進行驗證。我們的工廠利用DNA Dynamo進行此比對。SNAPGENE，一個免費的軟件程序可以打開這些文件進行查看。請參考我們的參考資料部分，以識別其他資源，這些資源可以幫助您檢查和操作所需的測序文件。提供了每種構建體的詳細數據表，其中列出了質粒的詳細信息以及的核苷酸和蛋白質序列。
請注意：我們的投資組合中有一部分構造可能沒有完整的可用數據集。這些是較舊的克隆，可在設備中使用，而當時并不一定要存儲序列數據。對于要求使用這些較早構建體的任何研究人員，我們將自費對它們進行重新排序，并在發出該構建體之前生成數據表。
所有cDNA克隆都以10ul的體積提供，濃度在10-100 ng /微升之間（取決于拷貝數）-在您自己的實驗室中有足夠的數量用于轉化和繁殖每個構建體！
| Type | Gene Name | Plasmid | Genbank | Species | Tag | Vector Type | Price per aliquot |
|---|---|---|---|---|---|---|---|
| cDNA Clones | MRCKView Data | pcDNA FRT/TO HA MRCK beta 1-473 D218A | NM_006035.3 | HA | Mammalian | £110.00 | |
| cDNA Clones | UVRAGView Data | pcDNA5D FRT/TO 3xflag UVRAG S508A | Human | FLAG | Mammalian | £110.00 | |
| cDNA Clones | UVRAGView Data | pcDNA5D FRT/TO 3xFlag UVRAG S508A S509A | Human | FLAG | Mammalian | £110.00 | |
| cDNA Clones | (chicken) CDKL5View Data | pcDNA5D FRT/TO Chicken CDKL5 TEV GST 6His | Sequence provided by Michael Morgan | Chicken | GST | Mammalian | £110.00 |
| cDNA Clones | (chicken) CDKL5View Data | pEX Chicken CDKL5 TEV GST 6His | Sequence provided by Michael Morgan. | Chicken | GST, 6His | Bacterial | £110.00 |
| cDNA Clones | (TEV)-Ubiquitin-UbiquitinView Data | pGST-1-(blunt)TEV_diubiquitin | P62987 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | (TEV)-Ubiquitin-UbiquitinView Data | pSc-B-(blunt)TEV_diubiquitin | P62987 | Other | £110.00 | ||
| cDNA Clones | -View Data | pSuperior Neo GFP- shRNA-NegativeControl | - | GFP | Mammalian | £110.00 | |
| cDNA Clones | 132View Data | pGL4.13 132 comp (1 copy) | Mammalian | £110.00 | |||
| cDNA Clones | 13376836View Data | pCMV-HA 13376836 | NM_025232 | Human | HA | Mammalian | £110.00 |
| cDNA Clones | 13376836View Data | pGEX 1337836 | NM_025232 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 13376836View Data | TOPO-13376836 | Other | £110.00 | |||
| cDNA Clones | 13376836 (presenilin like)View Data | pGFP-HA 13376836 | NM_025232 | Human | GFP, HA | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 betaView Data | pcDNA5-FRT/TO-mCherry 14-3-3 beta | XM_017028039.1 | Human | mCherry | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 betaView Data | pEBG6P-1 14-3-3 beta | NM_139323.2 | Human | GST | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 betaView Data | pET28a 14-3-3 beta | NP_647539 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 betaView Data | pGEX6P-14-3-3 beta | NM_139323.2 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 beta (mouse)View Data | pBluescript KS+mouse 14-3-3 beta | NM_018753.6 GI:158508429 with silent change g588a | Mouse | Other | £110.00 | |
| cDNA Clones | 14-3-3 epsilonView Data | pBabe hygro FLAG-mCherry 14-3-3 epsilon | Human | FLAG, mCherry | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 epsilonView Data | pcDNA5-FRT/TO NRL-14-3-3 epsilon | Mammalian | £110.00 | |||
| cDNA Clones | 14-3-3 epsilonView Data | pcDNA5-FRT/TO-mCherry 14-3-3 epsilon | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pcDNA5-FRT/TO-mCherry 14-3-3 epsilon | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pEBG-6 14-3-3 epsilon | HSU54778 | GST | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 epsilonView Data | pET28a 14-3-3 epsilon | NP_006752.1 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 epsilonView Data | pGEX 14-3-3 epsilon Y211/K | GST | Bacterial | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pGEX 14-3-3 epsilon E183/K | GST | Bacterial | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pGEX 14-3-3 epsilon F199/Y | GST | Bacterial | £110.00 | ||
| cDNA Clones | 14-3-3 epsilonView Data | pGEX-6-14-3-3 epsilon | HSU54778 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 epsilonView Data | pIRES2-mCherry 14-3-3 epsilon/pEGFP | NM_006761.4 GI:195546907 | GFP | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 epsilonView Data | pJET-m-cherry 14-3-3 epsilon | Other | £110.00 | |||
| cDNA Clones | 14-3-3 epsilonView Data | TOPO-14-3-3 epsilon | HSU54778 | Human | Other | £110.00 | |
| cDNA Clones | 14-3-3 epsilon (mouse)View Data | pBluescript KS+ mouse 14-3-3 epsilon | NM_009536.4 GI:226874905 with silent changes c639t and t711c | Mouse | Other | £110.00 | |
| cDNA Clones | 14-3-3 etaView Data | Hygro-retrovir-FLAG-mCherry 14-3-3 eta | Human | FLAG, mCherry | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 etaView Data | pcDNA5-FRT/TO-mCherry 14-3-3 eta | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 etaView Data | pEBG6P-14-3-3 eta | NP_003396.1 | Human | GST | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 etaView Data | pET28a 14-3-3 eta | NP_003396.1 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 etaView Data | pET28a 14-3-3 sigma | NP_006133.1 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 etaView Data | pGEX6P-1-14-3-3 eta | NP_003396.1 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 etaView Data | pJET-m-cherry 14-3-3 eta (Bgl2-Xho1) | Other | £110.00 | |||
| cDNA Clones | 14-3-3 eta (mouse)View Data | pBluescript KS+ mouse 14-3-3 eta | NM_011738.2 GI:162951868 | Mouse | Other | £110.00 | |
| cDNA Clones | 14-3-3 gammaView Data | Hygro-retrovir-FLAG-mCherry 14-3-3 gamma | Human | FLAG, mCherry | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 gammaView Data | pcDNA5-FRT/TO-mCherry 14-3-3 gamma | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 gammaView Data | pEBG6P-14-3-3 gamma | AAH20963.1 | Human | GST | Mammalian | £110.00 |
| cDNA Clones | 14-3-3 gammaView Data | pET28a 14-3-3 gamma | AAH20963.1 | Human | 6His | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 gammaView Data | pGEX6P-1-14-3-3 gamma | AAH20963.1 | Human | GST | Bacterial | £110.00 |
| cDNA Clones | 14-3-3 gammaView Data | pJET-m-cherry 14-3-3 gamma | Other | £110.00 | |||
| cDNA Clones | 14-3-3 gamma (mouse)View Data | pBluescript KS+ mouse 14-3-3 gamma | NM_018871.3 GI:229577260 | Mouse | Other | £110.00 | |
| cDNA Clones | 14-3-3 sigmaView Data | Hygro-retrovir-FLAG-mCherry 14-3-3 sigma | Human | FLAG, mCherry | Mammalian | £110.00 | |
| cDNA Clones | 14-3-3 sigmaView Data | pcDNA5-FRT/TO-mCherry 14-3-3 sigma | mCherry | Mammalian | £110.00 | ||
| cDNA Clones | 14-3-3 sigmaView Data | pCMV5-FLAG 14-3-3 sigma | AF029081 | Human | FLAG | Mammalian | £110.00 |
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